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TN0001
IDTN0001
Type / formTALEN, dimer. (T-D)
More about TALEN forms
Target Full sitecGGAGCCATCTGGCCGGGTtggctggttataACCGCGCAGATTCTGTTCa
gCCTCGGTAGACCGGCCCAaccgaccaatatTGGCGCGTCTAAGACAAGt
LengthLeft+[Spacer]+Right: 18+[12]+18
Speiceshuman (Homo sapiens)
List EENs targeting genome of human
Gene / LocusNTF3 G
Location to gene5'-Up
TALENs Left site & RVDs
NN NN NI NN HD HD NI NG HD NG NN NK HD HD NN NK NN NG
  G  G  A  G  C  C  A  T  C  T  G  G  C  C  G  G  G  T  
Right site & RVDs
NN NI NI HD NI NK NI NI NG HD NG NK HD NN HD NK NN NG
  G  A  A  C  A  G  A  A  T  C  T  G  C  G  C  G  G  T  
TALEN framework
& linker peptide
+63, +28
More about TALEN frameworks & linker pepetides
FokI variantWT
List EENs with FokI variants WT   More about FokI variants
Construction method(N/A)
More about TALE construction methods
Effectiveness
(first reported)
Genome modification
method
NHEJ, NHEJ-ODC
More about modification methods
Efficiency+, mismatch-detection nuclease assay, in K562 cells.
More about efficiency detection methods
Heritability
Specificity
Comments"+95" showed no activity; "NT-L+28/NT-R+63" showed highest activity.
Deletion 152aa in N-terminus.
Reference (first reported)Miller et al., Nat Biotechnol., 2011 (21179091 R)
External database
& resource




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